兔肝细胞中与RHDV VP60 P2亚区相互作用蛋白质的筛选和初步鉴定

doi:10.11843/j.issn.0366-6964.2016.03.018

Abstract

Abstract:

According to reports，VP60 P2 subdomain of RHDV may play an important role in the viral infection． In this study，we used co-immunoprecipitation(Co-IP) with RHDV VP60 P2 expressed in E.coli as bait protein and mass spectrometry analysis to screen the RHDV interacting hepatocellular proteins.Laminin receptor （LamR），as receptors of many other viruses，was identified and verified as potential interacting partner of P2.LamR gene was amplified by RT-PCR from rabbit liver cells．The recombinant LamR protein was expressed in E.coli and used in GST pull-down to verify the interaction of LamR and P2．Here we screened 26 proteins as potential P2 interacting proteins.After verification，the protein LamR could interact with P2 directly.We found that RHDV VP60 P2 interacted with LamR which would contributed to further study of the infectious process and pathogenesis of RHDV．

CLC Number:

LI Teng，JIANG Ping，WANG Fang，SONG Yan-hua，HU Bo，FAN Zhi-yu，WEI Hou-jun，QIU Ru-long，LIU Xing. Screening and Identification of RHDV VP60 P2 Domain Interacting Proteins in Rabbit Liver Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 549-558.

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Screening and Identification of RHDV VP60 P2 Domain Interacting Proteins in Rabbit Liver Cells

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